Pregnenolone – Powder, 5 grams
$16.99
Pregnenolone is a pregnane-class steroid derived enzymatically from cholesterol via the cholesterol side-chain cleavage enzyme (CYP11A1), positioned as the common biosynthetic precursor for the entire downstream steroidogenic cascade. Research interest centers on its role both as a steroidogenesis intermediate and as… CAS: 145-13-1 | Formula: C21H32O2 | Content: 5 grams | Research use only | Third-party lab tested.
Buy Pregnenolone – Powder, 5 grams – Swiss Chems USA
Pregnenolone – Powder, 5 grams — Research Overview
Pregnenolone is a pregnane-class steroid derived enzymatically from cholesterol via the cholesterol side-chain cleavage enzyme (CYP11A1), positioned as the common biosynthetic precursor for the entire downstream steroidogenic cascade. Research interest centers on its role both as a steroidogenesis intermediate and as a neurosteroid with independent signalling activity in the central nervous system. Research applications include steroidogenic pathway studies, neurosteroid receptor pharmacology, and CYP11A1 enzyme kinetics research.
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- For Laboratory Research Use Only
Intended use: This material is supplied for laboratory and in-vitro research only. It is not a drug, food, or cosmetic and is not intended for human or animal consumption.
Pregnenolone – Powder, 5 grams Chemical Properties
| Property | Specification |
|---|---|
| Chemical Formula | C21H32O2 |
| Synonyms | pregn-5-en-3β-ol-20-one, 3beta-Hydroxypregn-5-en-20-one, Arthenolone, Pregnetan |
| Molar Mass | 316.5 g/mol |
| CAS Number | 145-13-1 |
| PubChem CID | 8955 |
| Total Compound Content | 5 grams |
| Shelf Life | 36 months |
Pregnenolone – Powder, 5 grams Research Applications
Pregnenolone is generated from cholesterol by CYP11A1 within mitochondria of steroidogenic tissue, representing the rate-limiting branch point from which all downstream steroid hormone classes — progestogens, glucocorticoids, mineralocorticoids, androgens, and estrogens — are ultimately derived through tissue-specific enzymatic pathways. Independently of its role as a biosynthetic intermediate, pregnenolone and its sulfate ester are studied as neurosteroids with reported direct modulatory activity at GABA-A and NMDA receptor complexes in neuronal tissue, a mechanism distinct from genomic steroid receptor signalling. Research models examine both the classical steroidogenic cascade (using steroidogenic cell lines such as adrenal or gonadal-derived cultures) and neurosteroid receptor-binding pharmacology in neuronal preparations. Independently third-party HPLC-tested; COA available per batch.
Pregnenolone – Powder, 5 grams Frequently Asked Questions
What is pregnenolone’s role as the common precursor in the steroidogenic biosynthetic cascade?
Pregnenolone is produced from cholesterol by the mitochondrial enzyme CYP11A1 (cholesterol side-chain cleavage enzyme), representing the first and rate-limiting committed step of steroidogenesis. From pregnenolone, tissue-specific enzymatic pathways branch toward progesterone and downstream mineralocorticoids/glucocorticoids, or toward dehydroepiandrosterone (DHEA) and downstream androgens/estrogens, depending on which steroidogenic enzymes are expressed in a given tissue. Research using pregnenolone as a substrate in steroidogenic cell models allows characterisation of which downstream enzymatic branch point is rate-limiting under specific experimental conditions.
How is pregnenolone studied as a neurosteroid independent of its role in classical steroidogenesis?
Pregnenolone and its sulfated derivative (pregnenolone sulfate) are investigated for direct, rapid modulatory effects at neuronal ligand-gated ion channels — including GABA-A and NMDA receptor complexes — that occur independently of the genomic nuclear steroid receptor pathway used by downstream steroid hormones. Electrophysiological assays (patch-clamp recording of GABA-A or NMDA receptor currents) in the presence of pregnenolone or pregnenolone sulfate are the standard method for characterising this neurosteroid activity, distinct from steroidogenic enzyme-kinetics approaches used to study its biosynthetic role.
What experimental models are used to study CYP11A1 enzyme kinetics with pregnenolone as the reaction product?
Steroidogenic cell lines (such as adrenocortical or Leydig cell-derived models) and isolated mitochondrial preparations expressing CYP11A1 are standard systems for studying cholesterol-to-pregnenolone conversion kinetics, typically using cholesterol substrate (often radiolabeled or isotope-labeled) and quantifying pregnenolone product formation by mass spectrometry or immunoassay over time. These models allow researchers to characterise how experimental conditions or co-treatments affect this rate-limiting steroidogenic step.
Research Use Disclaimer
All product information is provided for educational and laboratory research reference only. Any form of introduction into humans or animals is prohibited. Handle only in licensed research settings in accordance with applicable laws and institutional protocols.
| Weight | 0.17 lbs |
|---|
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